Background: Advanced glycation end products (AGEs) and free radicals are inflammatory mediators and are\nimplicated in many diseases such as diabetes, cancer, rheumatoid arthritis etc. Multi targeted poly herbal drug\nsystems like Nawarathne Kalka (NK) are able to quench the overall effect of these mediators as they contain good\ncombinations of phytochemicals that have least side effects in contrast to modern medicinal drugs. The objectives\nof this study were to evaluate phytochemical composition, free radical scavenging activity, cytotoxicity and the\ninhibitory action on the formation of AGEs by aqueous extract of NK.\nMethods: Total phenolic content (TPC) and total flavonoid content (TFC) were determined using Folin ciocalteu\nmethod and aluminium chloride assay respectively. Free radical scavenging activity was assessed by DPPH radical\nscavenging assay (DRSA), phosphomolybdenum reduction antioxidant assay (PRAA) and nitric oxide (NO) scavenging\nassay. Brine Shrimp Lethality (BSL) bioassay was performed as preliminary screening for cytotoxic activity. Inhibitory\naction on AGE formation was evaluated using fructose mediated glycation of bovine serum albumin using\nfluorescence spectroscopic method.\nResults: The TPC and TFC were 75.1 Ã?± 3.0 mg/g gallic acid equivalents and 68.7 Ã?± 7.8 mg/g epigallocatechin gallate\nequivalents. The DRSA yielded EC50 of 19.15 Ã?± 2.24 Ã?¼g mLâË?â??1 for NK. DRSA of NK extract was greater than butylated\nhydroxy toluene (EC50 = 96.50 Ã?± 4.51 Ã?¼g mLâË?â??1) but lesser than L-ascorbic acid (EC50 = 5.60 Ã?± 0.51 Ã?¼g mLâË?â??1). The total\nantioxidant capacity of NK as evidenced by PRAA was 106.4 Ã?± 8.2 mg/g L-ascorbic acid equivalents. NK showed EC50\nvalue of 99.3 Ã?± 8.4 Ã?¼g mLâË?â??1 in the NO scavenging assay compared to the standard ascorbic acid (EC50 = 7.3 Ã?± 0.\n3 Ã?¼g mLâË?â??1). The extract indicated moderate cytotoxic activity in the BSL bioassay. The extract showed effective\ninhibitory action on the formation of AGEs with EC50 values of 116 Ã?± 19 Ã?¼g mLâË?â??1, 125Ã?±35 Ã?¼g mLâË?â??1 and 84 Ã?±\n28 Ã?¼g mLâË?â??1 in data obtained over three consecutive weeks respectively. Comparatively the reference standard,\naminoguanidine at a concentration of 500 Ã?¼g mLâË?â??1 demonstrated 65 % inhibition on the formation of AGE after\none week of sample incubation.\nConclusions: The results proved the potential of NK as a free radical scavenger, moderate cytotoxic agent and an\ninhibitor on the formation of advanced glycation end-products
Loading....